论文部分内容阅读
Objective:To study the effect of Baiban Ointment on gene expression profile of vulvar lichen sclerosus before and after treatment, and to provide theoretical basis for the early diagnosis and clinical treatment of vulvar lichen sclerosus. Methods:Nine patients with vulvar lichen sclerosus diagnosed pathologically were selected as the study object, and the Baiban ointment was applied locally for 3 months. Gene chip technology was used to detect the vulva skin tissue in the same area before and after treatment, and the peripheral normal skin was used as the normal control group to analyze the change of gene expression profile. FC and P values were used as research indicators, and the standard of differential expression was FC value ≥ 1.5, P value < 0.05. The differentially expressed genes were screened, and GO analysis and KEGG database gene pathway analysis were carried out. Results:Compared with the normal control group, there were 22 differentially expressed genes in Group A before treatment, of which 21 were up-regulated and 1 was down regulated; compared with Group B before treatment, there were 23 differentially expressed genes, all of which were up regulated. The differentially expressed genes related to Baiban ointment treatment included TBK1、STAT1、ITGAM、VCAN、PRKACB、PROM1、PLAT、SERPINA1. Go analysis showed that the up-regulated differential genes were mainly concentrated in the extracellular exosome, cytosol, extracellular space, Golgi apparatus and other cellular component, using the biological process such as positive regulation of cell metabolism, signal transduction regulation, cell adhesion, etc., to play a role in protein binding, enzyme activity regulation and other molecular functions. KEGG signaling pathway showed that the differentially expressed genes were significantly enriched in Toll like receptor signaling pathway, Nod like receptor signaling pathway and Fc RI signaling pathway, all of which were up-regulated signaling pathways. Among them, Toll like receptor signaling pathway and Nod like receptor signaling pathway are most closely related to the disease. Conclusion:Baiban ointment may play a role in regulating metabolism, inflammation and immune response by regulating the expression of related genes, affecting the signal transduction such as Toll like receptor signal pathway. The pathway and the genes screened in this study will provide a direction for the future study of this disease.