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Activation of urokinase-type plasminogen activator (uPA) receptor (uPAR) has been implicated in the initiation of proteolytic, adhesion and signal transduction mechanisms that permit metastasis by colon and other cancers.uPA specifically binds to its receptor and provides an inducible and localised cell surface proteolytic activity.It catalyses the cleavage of the zymogen plasminogen to plasmin, which in turn either directly degrades extracellular matrix or activates other zymogen proteases.The uPAR is expressed in human cancer cells at the invasive front and in tumour-associated macrophages.There is a strong correlation between uPAR expression and the invasive cancer cell phenotype.To determine whether expression of the uPAR is essential to the processes of cancer progress and spread, antisense RNA, DNAzyme uPAR and SiRNA approaches were used in our and other laboratories.HCT 116 colon cancer cells were transfected with an expression vector containing a uPAR eDNA fragment in an antisense orientation.