【摘 要】
：
Current immunomagnetic enrichment (IE) method can only detect general epithelial antigens of circulating tumor cells (CTCs).Further characterization of the CTCs to provide specific information on the
【机 构】
：
Department of Clinica Oncology Faculty of Medicine Hong Kong Cancer Institute and Prince of Wales Ho
【出 处】
：
BIT‘s2nd Annual World Cancer Congess-2009 (2009第二届癌症大会)
论文部分内容阅读
Current immunomagnetic enrichment (IE) method can only detect general epithelial antigens of circulating tumor cells (CTCs).Further characterization of the CTCs to provide specific information on the tumor type is not possible.We attempted to overcome this drawback by developing the methodology for using a gastrointestinal specific anti-cytokeratin (CK) 20 antibody to detect CTCs in colorectal cancer (CRC) patients blood.The protocol was validated using a CRC SW480 cell line.The clinical significance of findings in CRC was investigated by detecting CK20-positive CTCs (CK20 pCTCs) in patients with CRC, other common cancers (OCC), colorectal adenoma (CAD), benign colorectal diseases (BCD) and normal subjects (NS).Moreover, the malignant nature of CK20 pCTCs was examined by comparing chromosome 17 aberration patterns with those from the corresponding primary tumors.
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