【摘 要】
：
During the past number of years, the effort of my team has been focused on the identification of genes involved in the malignant progression of prostate cancer.We have used the differential display me
【机 构】
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School of Cancer Studies Liverpool University UK
【出 处】
：
BIT‘s2nd Annual World Cancer Congess-2009 (2009第二届癌症大会)
论文部分内容阅读
During the past number of years, the effort of my team has been focused on the identification of genes involved in the malignant progression of prostate cancer.We have used the differential display method as our core technique to identify candidate genes expressed differentially between benign and malignant model cells.To increase the effectiveness of the original differential display, we have developed three approaches, named SDD (Anal.Biochem.1999, 269:201-204), CCGE (Nucleic Acids Res.1999, 27:912-914), and MDD (Anal.Biochem.2000, 287:334-337), respectively.With these approaches, we have successfully identified a large number of candidate genes through comparing expression profiles of benign and malignant epithelial cells.To verify the differences of the expression levels of candidate genes in a wider range of benign and malignant cell lines, we have developed a "reverse slot blotting" procedure which included an internal control marker and in which the entire eDNA population was radioactively labeled and used as probes to hybridize the candidate cDNA fragments.
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