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为了评估转基因抗草甘膦除草剂大豆的食用安全性,以20%的比例将转基因抗草甘膦除草剂大豆GTS40-3-2和其亲本非转基因大豆A5403豆粕分别添加到基础饲料中喂养两代Sprague-Dawley(SD)大鼠,采用定性、定量PCR和ELISA方法检测转基因大豆成分相关基因和蛋白在长期饲喂的大鼠体内代谢残留状况。结果表明,大鼠喂养转基因大豆豆粕后,除了大鼠肠粪和盲肠内容物检测到有转基因成分的残留,肠道菌群和实质脏器均未发现相关基因和蛋白。结果提示,长期饲喂转基因抗草甘膦除草剂大豆GTS40-3-2与亲本A5403大豆豆粕对SD大鼠具有同样的食用安全性。
In order to assess the food safety of the transgenic glyphosate-resistant herbicide soybean, the transgenic glyphosate resistant soybean GTS40-3-2 and its parental non-transgenic soybean A5403 soybean meal were respectively added to the basal diet at a ratio of 20% to feed two On behalf of Sprague-Dawley (SD) rats, qualitative and quantitative PCR and ELISA were used to detect the metabolism of gene and protein related to transgenic soybean in long-term fed rats. The results showed that after the transgenic soybean soybean meal was fed, no gene and protein were found in the gut flora and the visceral organs except for the residues of the transgenic components in the intestinal contents and intestinal contents of rats. The results suggest that long-term feeding of transgenic glyphosate-resistant soybean GTS40-3-2 and parental A5403 soybean meal on SD rats have the same food safety.