【摘 要】
：
背景与目的 氧化应激状态是指由于机体抗氧化能力减弱,自由基不能及时清除,导致细胞发生氧化损伤.已有报道氧化应激在多种疾病的发生发展中起重要作用.还原型谷胱甘肽(GSH)是机体重要的还原剂,能够直接或间接参与机体抗氧化系统.谷胱甘肽还原酶(GSR)能够将氧化型谷胱甘肽(GSSG)还原为GSH,由此可见GSR蛋白在机体发挥抗氧化作用中至关重要.自然环境下,多种因素均能够诱导机体发生氧化应激,因此,我们
【机 构】
：
中国医科大学医学遗传学教研室,沈阳 110001
【出 处】
：
中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议
论文部分内容阅读
背景与目的 氧化应激状态是指由于机体抗氧化能力减弱,自由基不能及时清除,导致细胞发生氧化损伤.已有报道氧化应激在多种疾病的发生发展中起重要作用.还原型谷胱甘肽(GSH)是机体重要的还原剂,能够直接或间接参与机体抗氧化系统.谷胱甘肽还原酶(GSR)能够将氧化型谷胱甘肽(GSSG)还原为GSH,由此可见GSR蛋白在机体发挥抗氧化作用中至关重要.自然环境下,多种因素均能够诱导机体发生氧化应激,因此,我们在细胞水平上研究多种刺激因素对GSR的影响.方法 利用NaCl(320μM 24h)、H202(500μM 1h,1mM 1h)、乙醇(200mM 24h)、LPS(10μg/mL 24h)等分别刺激肝癌细胞Bel-7402；Westem blot检测GSR蛋白表达水平；相关试剂盒检测GSR酶活性、氧化应激损伤标志物丙二醛(MDA),总抗氧化能力(T-AOC)等指标.结果 Westem blot结果显示,几种刺激因素里,乙醇下调GSR表达(66.7％,P=0.0005),NaC1微上调GSR表达；酶活性检测发现,在乙醇刺激下,GSR活性下降32.5％(P=0.025),其他各组GSR活性变化不明显；各实验组MDA增高,T-AOC下降,显示均出现不同程度的氧化应激损伤.结论 乙醇能够诱导GSR表达下降,活性减弱,而其他几种氧化应激刺激因素无明显效果,提示乙醇对GSR蛋白具有调控作用,为后续实验进一步研究这一作用途径提供基础.
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