Preparation of temperature-responsive cationic copolymer modified stationary phase used for separati

• 作 者: Xiao Liang Zongjian Liu Rongji Dai Fangfang Geng Yanli Liang Yulin Deng
• 机 构: School of Life Science, Beijing Institute of Technology, Beijing 100081, China
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年3期
• 关键词: N-isopropylacylamide diethylamino(ethyl methacrylate) temperature-responsive ade

　　Poly(N-isopropylacylamide) (PNIPAAm), a typical temperature-responsive polymer, exhibits a reversible hydrophilic-hydrophobic change in water at its lower critical solution temperature (LCST) of 32℃, which is widely used in various areas.Moreover, a new chromatographic stationary phase utilizing PNIPAAm has been developed for separating bioactive materials in aqueous mobile phase without organic phase.In our study, certain-density P(N-isopropylacylamide-co-diethylamino(ethyl methacrylate)-co-N-tert-butylacrylamide) P(NIPAAm-co-DEAEMA-co-tBAAm) modified stationary phase is prepared through a surface-initiated atom transfer radical polymerization (ATRP).The grafting ratio of the copolymers is 11.31% determined by thermogravimetric analysis (TGA).