【摘 要】
：
Immunoassays are widely used in many fields ranging from medical diagnostics to environmental monitoring.The principal advantage of immunoassays is the spec
【机 构】
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DepartmentofChemistry,TsinghuaUniversity,Beijing100084,China
【出 处】
：
2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
Immunoassays are widely used in many fields ranging from medical diagnostics to environmental monitoring.The principal advantage of immunoassays is the specificity of detection,which derived from the selection of antibody-antigen binding.Chemiluminescence enzyme immunoassay(CLIA)has been intensively used in antigen-antibody detection,due to the sensitivity of CLIA is much higher than that of the traditional enzyme linked immunosorbent assay(ELISA).CLIA is a clinical diagnostic method with the basic principle of radioimmunoassay.It possesses both the advantages of chemiluminescence and immunoassay with high sensitivity and high selectivity,respectively.In this presentation,we shall report a chemiluminescence enzyme immunoassay based on magnetic microparticles which were used for the determination different kinds of targets in different samples.A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and validity
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