【摘 要】
：
The cadherin/catenin complex is an important cell adhesion complex that been shown to have many important physiological functions during neural development and synaptic plasticity.In previous work,we
【机 构】
：
Institute of Neuroscience and Key Laboratory of Neurobiology, Shanghai Institute of Biological Scien
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
The cadherin/catenin complex is an important cell adhesion complex that been shown to have many important physiological functions during neural development and synaptic plasticity.In previous work,we showed that the cadherin/ catenin complex plays a critical role in mediating activity-dependent dendritic morphogenesis.Here,we use two different molecular manipulations to interfere with N-cadherin in hippocampal neurons,specifically,by transfecting neurons with a cDNA encoding the intracellular domain of N-cadherin [N-cad(intra)] or a N-cadherin RNA interference construct.Both of these molecular manipulations have similar morphological effects,in that they reduce total dendritic length and branch tip number.However,the effect of these manipulations on neuronal function,as detected by electrophysiological recording,is different.Neurons transfected with N-cad(intra) do not show any change in mEPSC amplitude or frequency,while neurons transfected with the N-cadherin RNA interference construct show a significant decrease in mEPSC amplitude and frequency.From these morphological and electrophysiological results,we conclude that although interfering with N-cadherin using N-cad(intra) or using the RNAi method have the similar morphological effects,the functional effects are significantly different.
其他文献
Electroacupuncture (EA) has been reported to reduce body weight in over weighted subjects both in animal experiments and clinical studies.In the present smdy this effect of EA was tested in lean anima
Humoral regulatory mechanisms of hormones secretion in the anterior pituitary (AP) are considered to be significant in neuroendocrinology.But some researches have showed that a considerable amount of
星形胶质细胞的免疫调控功能已受到广泛关注.不同状态(静息/活化)的星形胶质细胞可调节T细胞增殖和凋亡,并影响T细胞因子的分泌.Th1和Th2型细胞因子在CNS炎性疾病的发生发展过程中发挥不同角色,本研究利用IFN-γ刺激活化星形胶质细胞,观察其调节T细胞因子(Th1/Th2)的分泌.本实验采用ELISA的方法检测T细胞分泌的细胞因子IL-2、IFN-γ、IL-4和IL-10,明确Th1型和Th2型
Objective To provide proof of the relationship between insulin signal transduction pathway and the mechanism of AD (Alzheimers disease) by observing the coexistence of associated proteins of insulin s
The paraventricular nuclues of the hypothalamus (PVN) is a central site for the integration of the neuroendocrine system and the autonomic nervous system.The PVN appears to be an important regulatory
Objective To define the role of GST on the central regulation of BP,HR,and RSNA.Methods Microinjected GST into RVLM and recorded BP,HR and RSNA simultaneously by using a set of biological polygraph.Re
During the neuronal development,the tremendous but tightly regulated expansion of plasma membrane is required for neurite outgrowth and arborization.However the underlying mechanism which regulates th
Bone Marrow Stromal cells(BMSCs) are mesenchymal elements that normally provide structural and functional support for hematopoiesis.The cytokines and growth factors excreted by BMSCs consist the micro
Proper dendritic arborization is essential for establishing normal neuronal circuitry.Here we report a role of geranylgeranyltransferase Ⅰ (GGT),a prenyltransferase which mediates lipid modification o
To study the influence of glutamic acid (Glu) on the electric activities of arcuate nucleus (ARC) neurons in the normal rats and inflamed rats which were induced by complete Freunds adjuvant (CFA).The