Characterization of microRNAs expression profiling in one group of Chinese urothelial cell carcinoma

• 作 者: Chen yuhua Duan xiuzhi Han xiucui Tao zhihua Wang siqi Wu xiuling Shen mo Chen hanguo Chen xiaogang Liu yongxia Zhu xiaoli Guo fei
• 机 构: Clinical Laboratory,The Fist Affiliated Hospital, Wenzhou Medical College,96 Fu Xue Lane,Wenzhou,Zhe
• 出 处: 中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议
• 发表日期: 2011年5期

@@ The goal ofthis study was to extend the known repertoire of microRNAs(miRNAs) expressed in urothelial bladder cancer(BCa)of the Chinese population and further understand the molecular events of miRNAs underlying urothelial bladder tumorigenesis at the global genome level.Materials and Methods We separated well-characterized epithelial tulnor cells from 20 moderately differentiated or poorly differentiated BCa specimens by laser capture microdissection (LCM)and pooled these cells of interest prior to RNA analysis.And 1 0 normal bladder epithelia (NBE)samples were pooled as the contr01.After preparation of small RNAs library，the two samples were sequenced simultaneously by the next generation high through-put Solexa sequencing technology.