Molecular engineering of enzymes to serve as ELISA labels for the enhanced quantification sensitivit

• 作 者: Fei LIAO Xiaolan YANG Chun ZHANG Hongbo LIU
• 机 构: Key Laboratory of Medical Laboratory Diagnostics (the Ministry of Education)and the Department of La
• 出 处: 中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议
• 发表日期: 2011年5期

@@ Enzyme-linked immunosorbent assay(ELISA) iS routinely used for the selective assay of a hapten， all antigen(Ag)，or an antibody(Ab)in specimens. The quantification sensitivity of ELISA is by no means satisfactory，however.The principal external factors affecting ELISA sensitivity include the number of an enzyme conjugated to all Ab，the catalytic eHlclency ot tlae conjugate，me reacuon period of the alzyme on its substrate and the sensitivity to quantify enzyme product(s).For practical efficiency，the reaction period is limited.For the reactivity to all analyte of interest，the number of the enzyme conjugated to the Ab is linlited.Therefore.the catalytic efficiency of alzynle·Ab conjugates，together with the sensitivity to quantify enzyme products，should be improved primarily.