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  5. Measurement of activities of enzyme labels in ELISA via an integration strategy to expand the quanti

Measurement of activities of enzyme labels in ELISA via an integration strategy to expand the quanti

来源 :中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议 | 被引量 : 0次 | 上传用户：q157194179
【摘 要】
：
@@ Enzyme-linked immunosorbent assay(ELISA) usually tolerates a nalTOW range to quantify an analyte of interest.In routine practice。activities of enzymes as labels in ELISA ale exclusively measured by
【作 者】
：
Xiaolan YANG Chun ZHANG Hongbo LIU Fei LIAO 
【机 构】
：
Key Laboratory of Medical Laboratory Diagnostics (the Ministry of Education) and the Department of
【出 处】
：
中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议
【发表日期】
：
2011年5期
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@@ Enzyme-linked immunosorbent assay(ELISA) usually tolerates a nalTOW range to quantify an analyte of interest.In routine practice。activities of enzymes as labels in ELISA ale exclusively measured by the classical initial rate method using synthetic chromogenic substrates.Due to potential interference from contaminants in these chromogenic substrates，their cost and potential substrate.inhibition.practical substrate levels are always lower than those for saturating enzyme labels in ELISA.Therefore，ELISA inherently has a narrow quantifiable range of an analyte of interest.
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