Peripheral Blood Gene Expression Profiles in Patients with Primary Biliary Cirrhosis

• 作 者: Lei Zhang Yongzhe Li Danxu Ma Xi Li Qun Shi Xin You Xiaomei Leng Mengtao Li Fulin Tang Fengchun Zhang
• 机 构: Department of Rheumatology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences
• 出 处: 中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议
• 发表日期: 2011年5期

@@ Primary biliary cirrhosis is a chronic cholestatic liver disease characterized by damage to the biliary epithelial cells.The pathogenesis of PBC remains unknown.Studies in this field suggested that PBC seems likely to be a multi-stage process，and possibly a consensus multi-stage model is needed.Previous studies on gene expression profiling in primary biliary cirrhosis have exclusively focused on liver tissue or intrahepatic cells.Since the pathological process is systemic，other complementary studies in blood cells seemed to be reasonable.In this study.we used oligonucleotide microarrays to explore differentially expressed genes in peripheral blood mononuclear cells(PBMCs)of PBC patients，and analyzed the results by signaling pathways.We confLrmed the microarray results on the biological level by quantitative real time polymerase chain reaction(RTQ-PCR)in larger samples and compared the gene expression profiles between PBMCS and liver tissue.