A Novel Monoclonal Antibody Specific to Non-small Cell Lung Cancer Inhibits Tumor Cell Growth and Bl

• 作 者: Pan Shiyang Wang Fang Xu Ting Zhang Lixia Huang Peijun Xu Jian Zhao Chun Xia Wenying
• 机 构: National Key Laboratory for Laboratory Medicine of China
• 出 处: 中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议
• 发表日期: 2011年5期

@@ We aim to prepare and characterize a monoclonal antibody(McAb)against human non-small cell lung cancer(NSCLC)，and to further investigate the inhibitory effect ofthis McAb on the proliferation of lung adenocarcinoma eell lineSPC.A1 in.vitro and in-vivo.BALB/c mice were immunized with human lung adenocarcinoma cells and the spleen cells of the immunized mice were conventionally fused with SP2/0 myeloma cells.The resulting positive clone，which produced McAb against SPC-A1.was obtained by means of indirect cell ELISA screemng.and the specificity of the McAb was indicated through ELISA and an indirect immunofluorescence assay.The relative molecular mass(Mr)of the antigen recognized by the McAb was measured by Westem blot and immunohistochemical analysis was performed to detect the tissue specificity of the McAb.SPC-A1 cells were plated on a soft agar matrix and treated with various concentrations of McAb;the colony formation efficiency and the inhibition ratio of colonies were then calculated.The xenograft was established upon subcutaneous injection of SPC-A1 cells and McAb，administered intraperitoneally at three difierent doses.The mice were monitored for tumor size.