A NSLC Cancer Project: From Sequence of Tumor Liberated Protein (TLP) to Function and Potential Diag

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  From the first analysis of immuneprecipitation followed by Western blotting Corin and TLP seem to precipitate at the same height (approximately 50KDa) and are recognized by the same antibodies.In parallel we are improving the tests of immunoprecipitation by the use of cell extracts derived from lung cancer cells A549 and NC1-H23 with the aim to be able of obtaining a precipitate containing only the TLP This result would allow a better sequence of the aminoterminal fragment of TLP and furthermore would allow to look in details the homologies between TLP and Corin.
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