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The effects of Cu2+ and pH on osteoclastic bone resorption lacunae are studied by culturing Japanese white rabbit osteo-clasts on bone slices. The number and surface area of bone resorption lacunae are measured by photomicrography and image analysis. Con-centration of calcium ion in the supernatant is measured by atomic absorption spectrometry to evaluate the activity of osteoclasts. The mor-phology of the lacunae is observed under a scanning electron microscope. The results indicate that Cu2+ at concentration of 1 . 00×10 6 mol/L and 1. 00×10- 7 mol/L inhibits osteoclastic activity and causes a dose-dependent reduction in the number and surface area of the lacunae. While the number of lanunae is increased and osteoclastic bone resorbing function is .significantly improved at Cu2+ concentra-lion of 1 . 00 × 10 8 mol/L. It is suggested that the effect of Cu2+ on osteoclastic bone resorption depends on Cu2+ concentration. pH had no significant effect on osteoclastic function in the near neutral range.
The effects of Cu2 + and pH on osteoclastic bone resorption lacunae are studied by culturing Japanese white rabbit osteo-clasts on bone slices. The number and surface area of bone resorption lacunae are measured by photomicrography and image analysis. Con-centration of calcium ion in the supernatant is measured by atomic absorption spectrometry to evaluate the activity of osteoclasts. The mor-phology of the lacunae is observed under a scanning electron microscope. The results indicate that Cu2 + at concentration of 1.00 × 10 6 mol / L and 1.00 × 10- 7 mol / L inhibits osteoclastic activity and causes a dose-dependent reduction in the number and surface area of the lacunae. While the number of lanunae is increased and osteoclastic bone resorbing function is. Significantly improved at Cu2 + concentra- lion of 1 00 × 10 8 mol / L. It is suggested that the effect of Cu2 + on osteoclastic bone resorption depends on Cu2 + concentration. PH had no significant effect on osteoclastic function in the ne ar neutral range.