Partial protection induced by phage library-selected peptides mimicking epitopes of Schistosoma ja

• 作 者: 欧阳理 易新元Department of Pathogen Biology 曾宪芳Department of Pathogen Biology 周金春Department of Pathogen Biology 王庆林Department of Pathogen Biology Larry Mc Reynolds
• 机 构: Department of Pathogen Biology,New England Biolabs Central South University Xiangya School of Medi
• 出 处: Chinese Medical Journal
• 发表日期: 2003年01期
• 关键词: Schistosoma phage library

Objective To obtain peptide mimicking epitopes of Schistosoma japonicum (S.japonicum) through screening of a phage peptide library and to test their potential for induction of protection. Methods S.japonicum infected sera from Microtus fortis (IMFS) and normal sera from Microtus fortis (NMFS) were used respectively to screen a 12-mers random peptide library by testing the reactivity of anti-S.japonicum serum with the phagotopes. After three rounds of biopanning, the pooled phages were used to immunize mice, after which challenge infection was performed. Results Of 12 randomly picked clones, 10 clones selected using IMFS and 7 clones selected using NMFS were shown to be antigenic. Significant reduction in adult worms (22.6%) and a high reduction (68.9%) in liver eggs were achieved following immunization with phages screened with IMFS. However, no protection was elicited by those selected with NMFS. Conclusion The results show that the phagotopes are both antigenic and immunogenic, suggesting a potential use of phage displayed peptide as novel vaccines against S. japonicum.

Objective To obtain peptide mimicking epitopes of Schistosoma japonicum (S. japonicum) through screening of a phage peptide library and to test their potential for induction of protection. Methods S. japonicum infected sera from Microtus fortis (IMFS) and normal sera from Microtus fortis ( NMFS) were used respectively to screen a 12-mers random peptide library by testing the reactivity of anti-S. japonicum serum with the phagotopes. After three rounds of biopanning, the pooled phages were used to immunize mice, after which challenge infection was performed Significant reduction in adult worms (22.6%) and a high reduction (68.9%) in liver eggs were achieved following immunization with phages screened with IMFS. However, no protection was elicited by those selected with NMFS. Conclusion The results show that the phagotopes are both antigenic and immunogenic , suggesting a potential use of phage displayed peptide as novel vaccines against S. japonicum.