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目的:①探讨氧化应激在醛同酮(ALDO)诱导的肾小球系膜细胞增殖中的作川;②探讨过氧化物酶体增殖物活化受体γ(PPARγ)激动剂对醛固酮诱导的肾小球系膜细胞增殖的抑制作用。方法:体外培养小鼠肾小球系膜细胞.应用~3H-胸腺嘧啶(~3H-TdR)掺入法、细胞计数及流式细胞术测定系膜细胞增殖和细胞周期的变化:应用Western blot检洲细胞周期素cyclin D和cyclin A表达;应用荧光探针2,7-二氯二氢荧光素乙酰乙酸(DCFDA)检测细胞内活性氧的变化。结果:①PPARγ受体激动剂罗格列酮可呈剂量依赖性的抑制醛同酮诱导的系膜细胞增殖,其抑制率可达80%以上:②醛固酮显著增加S期和G_2/M细胞数,该作用可被罗格列酮阻断;③罗格列酮可呈剂量依赖性的抑制醛同酮诱导的系膜细胞cyclin D和cyclin A表达;④抗氧化剂乙酰半胱氨酸(NAC)可显著抑制醛固酮诱导的系膜细胞增殖,罗格列酮可呈刹量依赖性的抑制醛固酮诱导的系膜细胞活性氧(ROS)产生。结论:氧化应激参与醛固酮诱导的系膜细胞增殖,PPARγ激动剂通过抑制氧化心激阻断醛固酮诱导的系膜细胞增殖。
To investigate the effects of peroxisome proliferator-activated receptor γ (PPARγ) agonist on aldosterone-induced aldosterone-induced proliferation of glomerular mesangial cells Inhibition of mesangial cell proliferation. Methods: Mouse mesangial cells were cultured in vitro and the proliferation and cell cycle of mesangial cells were measured by 3H-thymidine incorporation, cell counting and flow cytometry. Western blot The expression of cyclin D and cyclin A was detected in Chaochi, and the change of intracellular reactive oxygen species (ROS) was detected by fluorescent probe 2,7-dichlorodihydrofluorescein acetoacetate (DCFDA). Results: (1) Rosiglitazone, a PPARγ receptor agonist, inhibited the proliferation of mesangial cells induced by aldosterone in a dose-dependent manner with the inhibitory rate of more than 80%. ② Aldosterone significantly increased the number of S phase and G 2 / M cells, Rosiglitazone could inhibit the aldosterone-induced cyclin D and cyclin A expression in a dose-dependent manner. (4) The antioxidative agent acetylcysteine (NAC) Significant inhibition of aldosterone-induced mesangial cell proliferation, rosiglitazone in a dose-dependent manner inhibition of aldosterone-induced mesangial cell reactive oxygen species (ROS) production. CONCLUSION: Oxidative stress is involved in aldosterone-induced mesangial cell proliferation. PPARγ agonist blocks aldosterone-induced mesangial cell proliferation by inhibiting oxidative stress.