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利用差异显示ＰＣＲ 技术（ＤＤ＿ＰＣＲ） 比较了籼稻（ Ｏｒｙｚａｓａｔｉｖａ Ｌ．ｓｓｐ．ｉｎｄｉｃａ）“窄叶青８ 号”幼苗在盐胁迫与正常生长条件下基因表达的差异，克隆了水稻翻译延伸因子１Ａ 蛋白（ｅＥＦ１Ａ） 基因家族中的一个新的成员（ 称为ＲＥＦ１Ａ） 。利用Ｎｏｒｔｈｅｒｎ 杂交对环境因子胁迫下该基因的表达进行了分析，结果翻译延伸因子１Ａ 基因在水稻幼苗中的表达明显受盐胁迫和ＡＢＡ胁迫所诱导，且ＡＢＡ 胁迫处理对该基因转录的诱导要早于盐胁迫的诱导作用。此外，干旱（１５％ ＰＥＧ６０００） 处理、冷胁迫（４ ℃）和热激（３７ ℃） 对水稻翻译延伸因子１Ａ 基因的转录均有明显的诱导作用。上述结果说明翻译延伸因子１Ａ基因的诱导表达可能是水稻细胞对逆境胁迫的一种适应性反应
The differential expression PCR (DD_PCR) method was used to compare the gene expression of Oryzasativa L.ssp.indica seedlings under salt stress and normal growth conditions. The rice elongation factor 1A protein (eEF1A ) A new member of the gene family (called REF1A). Northern blot analysis of the expression of this gene under environmental stress resulted in the expression of the elongation factor 1A gene in rice seedlings significantly induced by salt stress and ABA stress and ABA stress induced earlier transcription of the gene Induction of salt stress. In addition, drought (15% PEG6000) treatment, cold stress (4 ° C) and heat shock (37 ° C) significantly induced transcription of the transcription elongation factor 1A gene in rice. The above results indicate that the induction of the expression of the elongation factor 1A gene may be an adaptive response of rice cells to stress