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大花鼠的新生乳鼠刚出生头一天和第三天,即予皮下各注射一剂麸氨酸(monosodium L-glutamate 4mg/gm b.w.),待鼠只成长为四月大时,自左心室灌流含有戊二醛2.5％,对甲醛2％与氯化钙0.05％的0.1M的砷酸缓冲液,酸碱度为7.4,然后取视神经及眼球组织块用锇酸处理,脱水及塑胶包埋剂包埋,半薄切片用甲苯蓝染色后用光学镜观察之,而超薄切片则用柠檬酸铅染色然后用电子镜观察,并以形态计量学研究之。正常对照鼠的视神经直径为0.54±0.06mm(X±S.E.,N=10),而麸氨酸单钠处理鼠的视神经直径减小至0.26±0.03mm(N=10),
Newborn rat born rat newborn rats were born the first day and the third day, each subcutaneous injection of a dose of glutamic acid (monosodium L-glutamate 4mg / gm bw) until the rats grew to April, from the left ventricle Perfusion with glutaraldehyde 2.5%, 2% formaldehyde and calcium chloride 0.05% 0.1M arsenate buffer, pH 7.4, and then take the optic nerve and ocular tissue block with osmium tetroxide, dehydration and plastic embedding package Buried, semi-thin section with toluene blue stained with optical microscopy, and ultra-thin sections are stained with lead citrate and then observed with electron microscopy, and morphometric studies. The optic nerve diameter of normal control mice was 0.54 ± 0.06 mm (X ± S.E., N = 10), while the optic nerve diameter of mice treated with monosodium glutamate decreased to 0.26 ± 0.03 mm (N = 10)