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nyc3基因在叶绿素降解过程中起关键作用。本研究通过分析nyc3基因在533份水稻核心种质材料中存在的自然变异,发现了nyc3基因序列具有非常丰富的单核苷酸多态性,并在基因的转录区域共找到了28个SNPs,其中在启动子区域检测到10个SNPs,在5’UTR检测到2个SNPs,在3’UTR检测到16个SNPs。利用生物信息学软件,将核心种质中nyc3分为14个单倍型,筛选其中6个主要单倍型进行染色体画图,明确了各单倍型之间存在的SNP差异,同时确定28个SNPs在基因转录区域的分布。通过实验室构建的全基因组范围内水稻全生育期表达谱数据库,找到在不同时期、不同组织中nyc3的表达量,通过分析可以发现,nyc3的表达量伴随着水稻的生长在不断的升高,在水稻成熟期,叶片中nyc3的表达量达到最高值,充分说明nyc3在调控叶绿素降解上起到作用。
The nyc3 gene plays a key role in chlorophyll degradation. In this study, we analyzed the natural variation of nyc3 gene in 533 rice core collection materials and found that the nyc3 gene sequence has a very rich single nucleotide polymorphism, and found a total of 28 SNPs in the gene transcription region, Among them, 10 SNPs were detected in the promoter region, 2 SNPs were detected in 5’UTR and 16 SNPs were detected in 3’UTR. Using bioinformatics software, nyc3 in core collection was divided into 14 haplotypes and 6 major haplotypes were screened for chromosomal mapping. The SNP differences among haplotypes were identified, and 28 SNPs Distribution in the gene transcription area. The expression level of nyc3 in different tissues and tissues was found in the genome-wide expression database of whole-growth period in the laboratory. Through the analysis, we found that the expression of nyc3 was continuously increased along with the growth of rice, At the rice ripening stage, the expression of nyc3 in leaves reached the highest value, which fully demonstrated that nyc3 plays a role in the regulation of chlorophyll degradation.