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将出生后1～5天的Wistar大鼠,在无菌条件下开颅取出小脑,经Hank液漂洗2次,剔除表面脑膜,尽可能刮去髓质,剩余的皮质充分剪碎,铺放在培养瓶底。待贴壁后加完全199(含小牛血清10％,常规用量的青霉素、链霉素),放进CO_2培养箱内培养。培养24小时后,可见细胞生长,继而大部分细胞发出长短不等的突起;少数细胞具有短而密的树突及一条长的轴突,此类细胞较肯定为神经细
The Wistar rats, 1 to 5 days after birth, were removed by craniotomy under aseptic conditions and rinsed twice with Hank’s solution. The surface meninges were removed and the medulla was scraped as much as possible. The remaining cortex was sufficiently sheared and placed on Training bottle bottom. After adherent to be added completely 199 (containing 10% calf serum, the conventional amount of penicillin, streptomycin), into CO 2 incubator culture. After culturing for 24 hours, cell growth was observed, and most of the cells emitted protrusions with different lengths. A few cells had short and dense dendrites and a long axon. Such cells were definitely more neurotic