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目的研究人多发性骨髓细胞系RPMI8226暴露于不同浓度氟化钠时细胞增殖、凋亡和周期的变化。方法采用CCK-8法检测不同剂量的氟化钠对RPMI8226细胞存活率的影响;采用Annexin V/PI流式细胞仪定量检测凋亡测定细胞周期和凋亡。结果氟化钠浓度为5、10、20、40、80和160μmol/L时细胞存活率均高于空白对照组,氟化钠浓度在320、640、1280和2560μmol/L时细胞存活率低于空白对照组,氟化钠浓度为10、20、40、80、160、320、640、1280和2560μmol/L时所测反映细胞增殖的吸光度(A)值差异均有统计学意义(P<0.05);细胞周期实验结果显示在不同剂量(5、40、320和2560μmol/L)时,随着氟化钠浓度的增加,G2期细胞相对数量减少,G1期细胞相对数量增多,就整个细胞周期而言,S期细胞相对数量比G1期、G2期均高,其中对照组与染毒组的相对细胞数量差异有统计学意义;细胞凋亡实验显示氟化钠浓度为320和2560μmol/L时均诱导细胞凋亡,其中细胞凋亡率在对照组与染毒组之间差异具有统计学意义(P<0.05)。结论氟化钠浓度为5、10、20、40、80和160μmol/L时促进RPMI8226细胞的增殖。氟化钠浓度在320、640、1280和2560μmol/L时抑制RPMI8226细胞的增殖。细胞周期和细胞凋亡实验显示DNA损伤程度与S期细胞相对数量一致,氟化钠对RPMI8226细胞造成的DNA损伤可能是细胞滞留在S期的一个重要原因。
Objective To study the changes of cell proliferation, apoptosis and cycle in human multiple myeloid cell line RPMI8226 exposed to different concentrations of sodium fluoride. Methods CCK-8 assay was used to determine the effect of different doses of sodium fluoride on the survival rate of RPMI8226 cells. The apoptosis rate was determined by Annexin V / PI flow cytometry. Results The cell viabilities of sodium fluoride at 5, 10, 20, 40, 80 and 160 μmol / L were all higher than those of the blank control group. The cell viability at sodium fluoride concentrations of 320, 640, 1280 and 2560 μmol / L was lower than In the blank control group, the differences of absorbance (A) between cell proliferation detected by sodium fluoride at 10, 20, 40, 80, 160, 320, 640, 1280 and 2560 μmol / L were all statistically significant ). The results of cell cycle assay showed that the relative number of cells in G2 phase decreased and the relative number of cells in G1 phase increased with the increase of sodium fluoride concentration at different doses (5, 40, 320 and 2560 μmol / L) However, the relative numbers of S phase cells were higher than those of G1 phase and G2 phase, and there was significant difference between the control group and the exposed group in the number of relative cells. The apoptotic experiments showed that sodium fluoride concentration was 320 and 2560μmol / L All induced apoptosis. The rate of apoptosis in the control group and the exposure group was statistically significant (P <0.05). Conclusion Sodium fluoride at 5, 10, 20, 40, 80 and 160 μmol / L can promote the proliferation of RPMI8226 cells. Inhibition of RPMI8226 cell proliferation at sodium fluoride concentrations of 320, 640, 1280 and 2560 μmol / L. Cell cycle and apoptosis experiments showed that the degree of DNA damage is consistent with the relative number of S phase cells. DNA damage induced by sodium fluoride on RPMI8226 cells may be an important reason for the cells staying in S phase.