论文部分内容阅读
Previously, we found a novel gene, nuclear receptor interaction protein (NRIP/DCAF6) that functions as a transcription cofactor for nuclear receptor-mediated gene expression.Here, we extensively deliberated NRIPs role in human papillomavirus (HPV) 16 gene expression.Firstly we found a novel glucorticoid response element (GRE) located at 7695 to 7710 bp in HPV 16 promoter except the previously reported three GRE sites responding glucorticoid.Secondly, NRIP enhances GR-regulated HPV-16 gene expression in the presence of hormone and NRIP associating GR only locating at GRE3 to activate HPV-16 promoter activity.Thirdly, virus E2 as a transcription cofactor can interact with GR to activate HPV-16 promoter activity in the presence of hormone and GRE3 site is also responsible for this induction in transient transfection assay.Fourthly, when introducing E2 into SiHa cells containing the integrated HPV-16 genome, it can stimulate E6/E7 gene expression in the presence of hormone but not absence that is correlated with p21 gene repression.Fifthly, NRIP can directly interact with E2 to induce HPV promoter activity regardless of hormone.Sixthly, NRIP and GR are new E2 binding proteins to regulate HPV gene expression.In sum, we propose a model that the complex formation between GR, NRIP and E2 locating at either GRE3 or E2 binding site in HPV-16 promoter to regulate vires gene expression.