【摘 要】
：
目的:探讨IL-25对小鼠气道周围新生血管形成的影响及机制方法:建立周期为70天的慢性哮喘小鼠模型,设立生理盐水组;50 μg OVA激发组;IL-25激发组.ELISA法检测bFGF、VEGF水平;免疫组化法观察vWF+血管数目、ERG、EGF、IGF-1、endotholin-1、angiogenin、amphiregulin等指标的变化;体外细胞实验研究IL-25通过作用于人脐静脉内皮细胞(
【机 构】
：
首都医科大学附属北京同仁医院呼吸科 首都医科大学免疫学系
论文部分内容阅读
目的:探讨IL-25对小鼠气道周围新生血管形成的影响及机制方法:建立周期为70天的慢性哮喘小鼠模型,设立生理盐水组;50 μg OVA激发组;IL-25激发组.ELISA法检测bFGF、VEGF水平;免疫组化法观察vWF+血管数目、ERG、EGF、IGF-1、endotholin-1、angiogenin、amphiregulin等指标的变化;体外细胞实验研究IL-25通过作用于人脐静脉内皮细胞(HUVEC)促进血管再生的功能.结果:和OVA激发组类似,IL-25组小鼠气道周围vWF+及ERG+血管数目显著增多,同时血管再生因子bFGF、VEGF、EGF、IGF-1、endothelin-1、angiogenin和amphiregulin表达增加;IL-25能够引起HUVEC增殖,并出芽生长,形成新生的微血管结构.
其他文献
ObjectivesThe recently discovered lnRNAs have the potential to regulate many biological processes.Our previous study showed that the IncRNA-GAS5 was decreased in lung cancer tissue, which contributed
Objectives: Transforming growth factor-beta1 and gene variants have been extensively studied in various human diseases.This Meta-analysis assessed association of TGF-β1-509C＞T [rs1800469],+869T＞C [rs
The effects of maternal influenza infection on the fetus are not well understood.To date there is little information regarding whether H7N9 influenza virus may be vertically transmitted across the pla
Background: Lung cancer is the leading cause of cancer-related mortality worldwide, with near 1.4 million deaths each year.NSCLC accounts for nearly 85％ of all cases of lung cancer.LncRNAs play import
Purpose: To investigate whether the over-expression of PRMT6 can attenuate the development of inflammation, apoptosis, oxidative stress in CSE-induced emphysema in a mice model.Methods: 32 BALB/c mice
Background: Increased apoptosis of pulmonary endothelial cells has emerged as a major mechanism in COPD development and progression.Notch signaling has been identified to play a critical role in the e
Background: Recent reports indicate that DNA methylation is regulated in part by the ERK pathway,and that ERK pathway is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD).Ou
目的:支气管扩张症在慢性阻塞性肺疾病(COPD)患者中呈现较高的发病率,COPD合并支扩患者的诊断和治疗较单纯COPD复杂,本研究将明确COPD合并支扩患者的临床特征,并阐述支扩对COPD患者预后的影响.方法:本研究回顾性收集2009年1月至2013年12月COPD患者临床相关指标及随访数据,运用相关统计学方法分析数据.结果:总共896例患者数据,其中合并支扩患者31 1例,多因素回归分析显示痰中
目的:线粒体融合基因-2 (mitofusin-2,Mfn2,又名增殖抑制基因,hyperplasia suppressor gene,HSG)编码的蛋白具有促进线粒体融合作用.该基因最早从自发性高血压大鼠和正常血压大鼠的血管平滑肌细胞中通过差异显示克隆获得,前期研究发现MFN2是高血压的致病基因,近年来有报道提示其与多种肿瘤相关.然而,MFN2在肺腺癌发生发展中的机制尚不明确,本研究拟通过细胞水
目的:探讨IL-25对小鼠气道周围纤维化的影响及机制.方法:建立周期为70天的慢性哮喘小鼠模型,设立①生理盐水组;②50μg OVA激发组;③IL-25激发组.ELISA法检测TGF-β1水平;免疫组化法观察成纤维细胞数目、细胞外基质、细胞外基质代谢相关酶类、CTGF等指标的变化;行肺功能检查评估小鼠呼吸参数改变.结果:和OVA激发组类似,IL-25组小鼠气道周围可见大量成纤维细胞增殖,伴随胶原Ⅰ