【摘 要】
：
目的 观察槲皮素-3-o-新橙皮糖苷对L6肌管细胞胰岛素抵抗模型葡萄糖消耗；脂肪酸消耗以及对PPARα；PPARγ； GLUT4表达量的调节作用及其作用机制.方法 以250 μmol·L-1的脂肪酸诱导形成L6肌管细胞胰岛素抵抗模型,以槲皮素-3-o-新橙皮糖苷干预24 h后,葡萄糖氧化酶法检测葡萄糖消耗量,气相色谱法检测脂肪酸消耗量,Wester蛋白质印迹法检测细胞中葡萄糖转运蛋白4(GLUT4
【机 构】
：
浙江省医学科学院,浙江杭州310013
论文部分内容阅读
目的 观察槲皮素-3-o-新橙皮糖苷对L6肌管细胞胰岛素抵抗模型葡萄糖消耗；脂肪酸消耗以及对PPARα；PPARγ； GLUT4表达量的调节作用及其作用机制.方法 以250 μmol·L-1的脂肪酸诱导形成L6肌管细胞胰岛素抵抗模型,以槲皮素-3-o-新橙皮糖苷干预24 h后,葡萄糖氧化酶法检测葡萄糖消耗量,气相色谱法检测脂肪酸消耗量,Wester蛋白质印迹法检测细胞中葡萄糖转运蛋白4(GLUT4)以及PPARα和PPARγ的表达量.结果 槲皮素3-o-新橙皮糖苷处理可提高L6细胞对葡萄糖的消耗(P＜0.01),降低细胞脂肪酸摄入(P＜0.05),上调GLUT4和PPARγ蛋白表达量(P＜0.01),但对PPARα的表达量无明显影响.结论 槲皮素-3-o-新橙皮糖苷可提高L6细胞胰岛素抵抗模型葡萄糖消耗量,降低脂肪酸摄入,调节PPARγ和GLUT4的表达,这些可能是受试物缓解胰岛素抵抗的机制之一.
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