RNAi induces innate immunity through multiple cellular signaling pathways

• 作 者: Zhongji Meng Xiaoyong Zhang Jun Wu Rongjuan Pei Yang Xu Dongliang Yang Michael Roggendorf Mengji Lu
• 机 构: Department of Infectious Diseases, Taihe Hospital, Hubei University of Medicine, Shiyan, China;Insti
• 出 处: 中华医学会第十六次全国病毒性肝炎及肝病学术会议
• 发表日期: 2013年7期

Background & Aims Our previous Conclusions showed that the knockdown of woodchuck hepatitis virus (WHV) by RNA interference (RNAi) led to upregulation of interferon stimulated genes (ISGs) in primary hepatocytes.In the present study, we tested the hypothesis that the cellular signaling pathways recognizing RNA molecules may be involved the ISG stimulation by RNAi.Methods Primary murine hepatocytes (PMHs) from wild type mice and WHV transgenic (Tg) mice were prepared and treated with defined siRNAs.The mRNA levels of target genes and ISGs were detected by real-time RT-PCR.The involvement of the signaling pathways including RIG-I/MDA5, PKR, and TLR3/7/8/9 was examined by specific inhibition and the analysis of their activation by Western blotting.