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The monitoring of autofluorescence in skin tissue samples can have diagnostic and therapy significance.In this study, we are the first to describe autofluorescence of eccrine sweat glands, which is important and helpful for the diagnosis and therapy of diseases that involve the eccrine sweat glands.The results indicated that sweat gland autofluorescence in Hematoxylin-eosin stained skin tissue sections is easily observed under a fluorescence microscope and appears green under BW and red under GW.Furthermore, a single eccrine sweat gland separated from a full-thickness skin graft was also evaluated.The single eccrine sweat gland showed various autofluorescence colours, including green under WB and red under WG.The autofluorescence intensity of the single eccrine sweat gland was measured using a laser confocal scanning microscope system.The spectrum excited at 488 nm exhibited two peaks located at approximately 530 nm (11.54±4.66) and 590 nm (10.38±4.33).The results suggest fiavin and lipopigment as the endogenous fluorophores.Based on our study, the autofluorescence of the HE stained eccrine sweat gland sections is simple and is helpful for easily determining the structure of eccrine sweat glands.The autofluorescence of the single eccrine sweat gland may be due to the existence of flavin and lipopigment.