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In the study, we adopted several methods, including ET-1 treatment and the control of the parameter change in the culture, to regulate the tissue engineered cardiac conduction bundle (TECCB) conduction velocity.After the conduction velocity regulation, we implanted TECCB into atrioventricular groove of rabbit hearts creating an AV conduction passway in an attempt to verify the effect.First, rat cardiac progenitor cells (rCPCs) were isolated from heart tubes and biological characteristics of the cells, including molecular expressions and growth properties, were investigated.Based on this, rCPCs were seeded into collagen sponge so as to construct TECCB.During the course of cultivation, ET-1 treatment and the control of the parameter change were used to regulate TECCB conduction velocity.After being treated with ET-1, rCPCs resided in TECCB underwent a phenotype change.The conduction velocity was not lower in TECCB treated with ET-1 than the untreated.Interestingly, we found that controlling the cultivation time and the seeding cells number could effectively regulate TECCB conduction velocity.In addition, we also found that the length could not influence on TECCB conduction velocity.Subsequently, we implanted TECCB(1 w, 106cells/ml)into atrioventricular groove of rabbit hearts creating an AV conduction passway to observe the effectiveness.The results showed that the mortality in sham operation group and blank scaffold group is significantly higher than that in transplantation group after atrioventricular block.In a week, the recovery is better in transplantation group than sham operation group and blank scaffold group.