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MethBank is a database that integrates whole-genome single-base nucleotide methylomes and provides an interactive browser for visualization of high-resolution DNA methylation data.Here we present an updated implementation of MethBank (http://bigd.big.ac.cn/methbank;Version 2.0)by incorporating high-quality whole-genome bisulfite sequencing methylome maps for five economically important crops (Oryza sativa,Glycine max,Mcmihot esculenta,Phaseolus vulgaris and Solanum lycopersicum) as well as two model animals (Danio rerio and Mus musculus).