【摘 要】
：
目的：比较生物发光和小动物PET/CT成像技术在BALb/c小鼠乳腺癌原位移植模型中的显像效果,寻找适合的评价乳腺癌肿瘤模型的分子影像学方法.方法：将表达荧光素酶基因的乳腺癌细胞4T1,接种于雌性BALB/e小鼠左侧第二对乳房垫下使其成瘤,活体荧光成像和小动物PET/CT成像观察肿瘤的生长情况.18F-FDG PET/CT显像利用IRW软件对图像进行分析,采用感兴趣区技术(ROI)测量组织18F-
【机 构】
：
中山大学附属第一医院, 广东广州510080
【出 处】
：
2016华南实验动物科学国际研讨会、第三届实验小型猪国际论坛暨广东·九州实验动物学术交流会
论文部分内容阅读
目的：比较生物发光和小动物PET/CT成像技术在BALb/c小鼠乳腺癌原位移植模型中的显像效果,寻找适合的评价乳腺癌肿瘤模型的分子影像学方法.方法：将表达荧光素酶基因的乳腺癌细胞4T1,接种于雌性BALB/e小鼠左侧第二对乳房垫下使其成瘤,活体荧光成像和小动物PET/CT成像观察肿瘤的生长情况.18F-FDG PET/CT显像利用IRW软件对图像进行分析,采用感兴趣区技术(ROI)测量组织18F-FDG摄取值.结果：活体荧光成像系统观察能够在短时间内特异性的探测到肿瘤组织,小动物PET/CT影像观察发现小鼠体内多数器官组织都有18F-FDG摄取,但肿瘤组织在所有组织、器官中18F-FDG摄取值最高,肿瘤/组织或器官的18F-FDG摄取比率较高.结论：对比两种成像技术,发现在本实验中小动物生物发光活体成像比小动物18F-FDG PET/CT特异性好,操作简单,图像采集时间短,价钱便宜,无放射性,可检测细胞存活状态,但小动物PET/CT分辨率较高,可精准定位,图像三维立体,可监测到细胞的代谢能力,为以后实验研究中正确选择活体影像学监测手段提供依据.
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