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Since Clack fabricated the first biosensor in the year 1962,various novel biosensors have been developed in lab for the detections of different physiological substances.However,in the clinic healthcare,rare prepared biosensors can be really applied due to the unsatisfied sensitivity,selectivity and high cost.In the last decade,the emerging nanomaterials have exhibited an evident improvement of the biosensing performance due to the high catalysis and conductivity.[1-3] But the common synthesis routes of nanomaterials cannot be developed in the large-scale manufacture,which hinders the product transformation in biosensor researches.Therefore,the design of low cost and large-scale approach for biosensing nanomaterial synthesis will be beneficial for the product development in the real application.In this presentation,in order to address above issue,we have developed a novel screen-printing strategy to realize the scaled fabrication of a Prussian blue(PB)biosensor chip with the nanocubic crystals.Through a low-speed chemical synthesis approach,the nanostructure of PB slurry was well controlled to be a 100 nm nanocube by adjusting the synthesis temperature and reactant concentration.To construct a intact microchip,Ag/AgCl and carbon inks were respectively served as the reference and counter electrodes.Under the low work potential of-0.05 V,due to the high electrocatalytic activity attributed by the regular nanostructure of PB,[4-5] the as-prepared chips exhibited the generally high sensitivities of 83.404,31.642 and 6.379 μA mM-1 cm-2 for the respective detections of glucose,glutamate and lactate.Besides,the fabricated biosensor showed excellent stability,good reproducibility and high selectivity.It is believed that the low-cost and mass production route would be promising in the construction of more advanced biosensors for the product development and be further applied in medical diagnosis.