【摘 要】
：
Fluorescent probes such as organic dyes and fluorescent proteins are widely used in modern biology and medicine.However,the intrinsic limitations of these p
【机 构】
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StateKeyLabonIntegratedOptoelectronics,CollegeofElectronicScienceandEngineering,JilinUniversity,Chan
【出 处】
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2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
Fluorescent probes such as organic dyes and fluorescent proteins are widely used in modern biology and medicine.However,the intrinsic limitations of these probes,such as low absorptivity and poor photostability,have posed great difficulties in further developments of high-sensitivity imaging techniques and high-throughput assays.Semiconducting polymer dots(Pdots)have recently been demonstrated for various biological imaging and sensing applications because of their superior fluorescence properties.[1-4] A general strategy has been developed for Pdot functionalization that allows for covalently linking Pdots to biomolecules.The Pdot bioconjugates can effectively and specifically label cellular targets,without any detectable non-specific binding.Single-particle imaging,cellular imaging,and flow cytometry experiments indicate that cellular labeling with green-emitting Pdots of~10 nm diameter are~30 times brighter than those of Alexa 488 dye and Qdot 565 probes.Here we present our recent advances in design and development of multifunctional Pdot probes for biological applications,such as 1)highly efficient Pdot photosensitizer for photodynamic therapy; 2)implantable Pdot transducers for small molecule detection; 3)highly bright Pdot probes for stem cell labeling and tracking.Our results indicate that Pdots are versatile and promising platforms for biomedical applications.
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