论文部分内容阅读
The basolateral amygdala is a brain region with critical roles in emotional control and fear conditioning.To understand the basolateral amygdala physiology and pathology,it is important to study its synaptic transmission and modulation.However,technical difficulties exist in dissecting basolateral amygdala regions.To overcome this problem,we have developed a novel dissection procedure in which we can gain the basolateral amygdala regions.Here,we describe a reliable method to prepare primary cultures of P0 rat basolateral amygdala neurons.Production of cultures can take as little as 2 h.Cells can be maintained for as long as 2 week.The neurons survived better and showed healthy morphology.Thus,the present study suggest that primary culture of the basolateral amygdala neurons is a powerful tool to study neuronal synaptic transmission and modulation.