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目的研究炭疽毒素受体1(anthrax toxin receptor 1,ANTXR1)对食管癌细胞系ECA109的迁移及侵袭能力的影响及机制。方法采用化学合成ANTXR1基因的小干扰RNA(small interfering RNA,si RNA)下调该基因表达,采用实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,RT-PCR)和Western blot检测转染后的细胞内ANTXR1、基质金属蛋白酶2(matirx metallo-proteinases 2,MMP 2)和基质金属蛋白酶9(matirx metallo-proteinases 9,MMP9)的表达,通过细胞划痕实验和Transwell侵袭实验分别检测ANTXR1对ECA109细胞迁移和侵袭的影响。结果将ANTXR1转染入ECA109后,细胞中ANTXR1 m RNA和蛋白水平都明显降低,MMP2、MMP9表达明显下调,ECA109细胞的迁移和侵袭能力显著降低,与对照组相比差异有统计学意义(P<0.01)。结论在食管癌细胞系ECA109中ANTXR1与细胞的迁移和侵袭能力相关,ANTXR1可能通过影响MMP2和MMP9的表达参与其中,有望成为食管癌早期诊断、分子治疗的靶点。
Objective To investigate the effect and mechanism of anthrax toxin receptor 1 (ANTXR1) on the migration and invasion of esophageal carcinoma cell line ECA109. Methods The mRNA expression of ANTXR1 gene was down-regulated by small interfering RNA (siRNA) of the ANTXR1 gene. The expression of the gene was detected by real-time polymerase chain reaction (RT-PCR) and Western blot The expression of ANTXR1, matirx metallo-proteinases 2 (MMP 2) and matirx metallo-proteinases 9 (MMP9) were detected by cell scratch assay and Transwell invasion assay. Effect of ECA109 cell migration and invasion. Results After transfection of ANTXR1 into ECA109 cells, the mRNA and protein levels of ANTXR1 were significantly decreased, the expressions of MMP2 and MMP9 were significantly down-regulated, and the migration and invasion ability of ECA109 cells was significantly decreased compared with the control group (P <0.01). Conclusion ANTXR1 is associated with cell migration and invasion in esophageal cancer cell line ECA109. ANTXR1 may be involved in the expression of MMP2 and MMP9, which may be the target of early diagnosis and molecular therapy of esophageal cancer.