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目的探讨血府逐瘀汤对非酒精性脂肪性肝病(NAFLD)大鼠肝脏X受体α(LXR-α)的影响。方法雄性SD大鼠,分为正常组10只,血府逐瘀汤组(治疗组)和模型组各20只。治疗组和模型组用高脂饲料喂养10周建立NAFLD模型。造模成功后,各组进行相应干预共6周。采用HE染色法观察大鼠肝组织病理变化,测定各组大鼠肝功能,并用荧光定量RT-PCR检测各组大鼠LXR-α基因表达。结果①与正常组比较,模型组血清ALT、TCH升高,差异有统计学意义(P<0.05)。与模型组比较,治疗组血清AST下降,差异有统计学意义(P<0.05)。血清TG、FFA各组间比较,差异无统计学意义(P>0.05)。②与正常组比较,模型组肝组织TG、TCH、FFA、LXR-αmRNA水平均显著升高,差异有统计学意义(P<0.01)。与模型组比较,治疗组肝组织LXR-αmRNA水平显著降低,差异有统计学意义(P<0.01);而TG、TCH、FFA差异无统计学意义(P<0.05)。结论血府逐瘀汤能降低NAFLD大鼠LXR-α的基因表达,降低肝脏损害。
Objective To investigate the effects of Xuefu Zhuyu Decoction on liver X receptor α (LXR-α) in non-alcoholic fatty liver disease (NAFLD) rats. Methods Male SD rats were divided into normal group (n = 10), Xuefu Zhuyu Decoction group (n = 20) and model group (n = 20). The treatment group and model group were fed with high-fat diet for 10 weeks to establish NAFLD model. After the success of modeling, each group for the corresponding intervention for 6 weeks. The pathological changes of rat liver were observed by HE staining. The liver function of rats in each group was measured. The expression of LXR-α gene in each group was detected by real-time fluorescence quantitative RT-PCR. Results ① Compared with the normal group, serum ALT and TCH in the model group increased significantly (P <0.05). Compared with the model group, serum AST decreased in the treatment group, the difference was statistically significant (P <0.05). Serum TG, FFA between the various groups, the difference was not statistically significant (P> 0.05). ②Compared with the normal group, the levels of TG, TCH, FFA and LXR-αmRNA in the model group were significantly increased, the difference was statistically significant (P <0.01). Compared with the model group, the level of LXR-αmRNA in the liver tissue of the treated group was significantly decreased (P <0.01), while there was no significant difference in TG, TCH and FFA (P <0.05). Conclusion Xuefu Zhuyu Decoction can reduce the gene expression of LXR-α in NAFLD rats and reduce the liver damage.