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以菜芙蓉花乙醇提取物为原料制备分离了石油醚(PE)相、乙酸乙酯(EA)相、正丁醇(BA)相和萃余水溶物(AQ)相4个不同活性部位。以谷胱甘肽过氧化物酶(GSH-Px)、ATP酶(ATPase)为指标,考察了各部位对FeSO_4和H_2O_2诱导小鼠肝匀浆氧化损伤的保护,对活性部位进行了HPLC分析并考察了活性最强部位抑制质粒DNA氧化损伤的作用。结果表明:在黄酮浓度为300 mg/L时,EA相提高GSH-Px活力达62.82 U/μg prot;在AQ相黄酮质量浓度为300 mg/L时,AQ能有效提高Na~+-K~+-ATPase活力达到270.85 U/μg prot,在AQ相黄酮质量浓度为600 mg/L时,AQ能有效提高Ca~(2+)-Mg~(2+)-ATPase活力达到232.88 U/μg prot。EA相含有多种黄酮类物质,其中金丝桃苷含量为116.19μg/g、杨梅素含量为67.70μg/g、槲皮素含量为29.13μg/g。EA相对DNA氧化损伤具有浓度依赖性的保护作用。EA相高抗氧化能力是由其分子组成及化学活性决定的。
Four different active sites of petroleum ether (PE) phase, ethyl acetate (EA) phase, n-butanol (BA) phase and aqueous extract of raffinate (AQ) The GSH-Px and ATPase were used as indexes to study the protective effect of different parts on oxidative damage of liver homogenates induced by FeSO 4 and H 2 O 2 in mice. The active sites were analyzed by HPLC The effect of inhibiting the oxidative DNA damage of plasmid DNA at the most active site was investigated. The results showed that the EA phase increased the activity of GSH-Px to 62.82 U / μg prot at the flavone concentration of 300 mg / L and AQ increased the Na ~ + -K ~ + -ATPase reached 270.85 U / μg prot. AQ could effectively increase the Ca 2+ (2+) - ATPase activity to 232.88 U / μg prot at the concentration of 600 mg / L AQ . EA contains a variety of flavonoids, of which hyperoside content of 116.19μg / g, myricetin content of 67.70μg / g, quercetin content of 29.13μg / g. EA has a concentration-dependent protective effect against DNA oxidative damage. The high antioxidant capacity of EA phase is determined by its molecular composition and chemical activity.