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目的探讨低频超声对大鼠实验性口腔黏膜溃疡愈合时间及溃疡组织中一氧化氮(NO)和一氧化氮合酶(NOS)含量的影响。方法健康Sprague-Dawley(SD)大鼠36只,雌雄各半,完全随机分为6组(n=6),1组设为正常对照组,其余5组利用氧自由基诱导法+物理烧伤法建立SD大鼠颊囊黏膜口腔溃疡动物模型,分别为溃疡组、药物组和低频超声组(1.1、1.58、2.06 W)。末次治疗24 h后观察溃疡愈合时间,并采用分光光度计测定大鼠口腔颊囊黏膜组织中NO、NOS含量。结果大鼠溃疡病理特点与人类口腔溃疡病理特点基本相似。低频超声组较溃疡组及药物组的溃疡愈合时间短。NO活性测定结果显示,低频超声组1.1、1.58、2.06 W[分别为(9.86±1.69)、(9.42±1.34)、(8.51±1.26)μmol/g]较溃疡组[(23.85±4.81)μmol/g]显著降低(P<0.05),与正常对照组(10.88±1.68)μmol/g比较无统计学差异(P>0.05);NOS活性检测结果同样表明,低频超声组1.1、1.58、2.06 W[分别为(3.89±0.72)、(4.35±0.53)、(4.74±0.81)μmol/g]较溃疡组[(6.48±0.77)μmol/g]显著降低(P<0.05),与正常对照组(3.83±0.99)μmol/g比较无统计学差异(P>0.05)。结论适合剂量参数的脉冲低频超声能有效降低溃疡组织中的NO、NOS活性含量,对溃疡的愈合有一定的促进作用。
Objective To investigate the effect of low frequency ultrasound on the healing time of experimental oral mucosal ulcer and the content of nitric oxide (NO) and nitric oxide synthase (NOS) in ulcer tissue in rats. Methods Thirty-six Sprague-Dawley (SD) rats were randomly divided into 6 groups (n = 6), one group was normal control group and the other 5 groups were treated by oxygen free radical induction and physical burn method Animal models of buccal mucosa bursal ulcer in SD rats were established, which were ulcer group, drug group and low frequency ultrasound group (1.1, 1.58, 2.06 W) respectively. The ulcer healing time was observed 24 h after the last treatment, and the contents of NO and NOS in oral cheek pouch mucosa were measured by spectrophotometer. Results The pathological characteristics of rat ulcer were similar to those of human oral ulcer. Low-frequency ultrasound group than the ulcer group and drug group ulcer healing time is short. The results of NO activity showed that in the low-frequency group, the values of 1.1, 1.58, 2.06 W [(9.86 ± 1.69), (9.42 ± 1.34), (8.51 ± 1.26) μmol / g] g, P <0.05). There was no significant difference between the two groups (P> 0.05), but there was no significant difference between them (10.88 ± 1.68) μmol / (3.89 ± 0.72), (4.35 ± 0.53) and (4.74 ± 0.81) μmol / g, respectively, compared with those in ulcer group [(6.48 ± 0.77) μmol / g] ± 0.99) μmol / g, there was no significant difference (P> 0.05). Conclusion Pulsed low-frequency ultrasound which is suitable for dose parameters can effectively reduce the content of NO and NOS in ulcer tissue and promote the healing of ulcer.