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牛磷酸作为强化保健食品的功效成份,根据国家《保健食品技术规范》要求,必须对其含量及稳定性进行监测.目前,国家尚无标准检测方法.采用反相高效液相色谱法,以邻苯二甲醛(OPA)为衍生剂进行柱前衍生测定.经实验证明该法适用于强化食品中的牛磺酸检测,方法回收率在88%～98%,相对标准偏差为3.1%.各种常见氨基酸及维生素等强化剂不干扰本测定.
As an effective component of fortified health food, bovine phosphoric acid must be monitored for its content and stability according to the requirements of the National Technical Code for Health Food. At present, there is no standard test method in the country. By using reversed-phase high performance liquid chromatography, Phthalaldehyde (OPA) as derivatizing agent for pre-column derivatization.It has been proved by experiments that this method is suitable for the determination of taurine in fortified food with the recovery of 88% -98% and the relative standard deviation of 3.1% Common amino acids and vitamins and other enhancers do not interfere with the determination.