论文部分内容阅读
以三七幼苗(30 d苗龄)为试验材料,研究了聚乙二醇(PEG 6000)模拟干旱胁迫对其各部位抗氧化酶、渗透物质和根系活力等生理指标的影响。结果表明,根和叶中POD,APX活力随处理浓度(PEG 6000 0,2.5%,5.0%,7.5%)和时间(1,2,3 d)的增加而不断上升,SOD则表现为在同一处理时间下随处理浓度的增加而先升后降,同一处理浓度下则随处理时间的延长而上升,CAT活性表现为在同一处理时间下随处理浓度的增加而增加,同一处理浓度下则随处理时间的延长而呈先升高后降低的趋势;茎中SOD,APX活性变化不明显,CAT活性随处理时间和浓度的增加而升高;根和叶中MDA、可溶性蛋白和脯氨酸含量及根系活力随处理浓度的升高和处理时间的延长而显著升高;处理1 d后,根尖H2O2和NO荧光信号随处理浓度的升高而增强。综上所述,三七幼苗能够通过调节抗氧化酶系统、渗透胁迫物质及抗逆胁迫信号物质应对干旱胁迫,当PEG6000超过5.0%(即溶液水势为-0.50 MPa)时可对三七幼苗产伤害,应及时补水抗旱。
The seedlings of Panax notoginseng (30 d seedling age) were used as experimental material to study the effects of PEG 6000 on the physiological indexes such as antioxidant enzyme, osmotic substance and root activity of various parts under simulated drought stress. The results showed that the activities of POD and APX in roots and leaves increased with the increase of treatment concentration (PEG 6000 0, 2.5%, 5.0%, 7.5%) and time (1, 2, 3 d) Under the same treatment concentration, the CAT activity increased with the increase of the treatment concentration at the same treatment time, but increased with the increase of the treatment concentration at the same treatment concentration Treatment time was prolonged and then decreased firstly; SOD and APX activities in stems did not change significantly, CAT activity increased with the increase of treatment time and concentration; MDA, soluble protein and proline content in roots and leaves And root vigor increased with the increase of treatment concentration and treatment time. After 1 day of treatment, the fluorescence signals of root tip H2O2 and NO increased with the increase of treatment concentration. In summary, the seedlings of Panax notoginseng can cope with drought stress by regulating the antioxidant enzyme system, osmotic stress substances and anti-stress stress signal substances. When the PEG6000 content exceeds 5.0% (ie, the solution water potential is -0.50 MPa) Injury, should replenish drought.