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目的探索丹酚酸B改善高脂饮食引起的小鼠牙槽骨骨量丢失的作用以及可能的作用机制。方法 C57BL/6J雄性小鼠30只,分为3组:正常组、高脂饮食组和丹酚酸B组,每组10只。除正常组外,其他两组给予高脂饮食,丹酚酸B组用丹酚酸B[125 mg/(kg·d)]干预,治疗12 w后,取小鼠牙槽骨,然后分别用双能X射线测量骨密度,HE染色观察骨微结构,免疫组织化学染色分析牙槽骨核因子-κB-p65(nuclear factor-kappa B,NF-κB-p65)、组织蛋白酶K(Cathepsin K)、超氧化物歧化酶(superoxide dismutase,SOD)和NADPH oxidase 4(Nox4)的表达情况。结果丹酚酸B能明显改善高脂饮食诱发的小鼠牙槽骨骨密度下降以及骨微结构破坏。同时,丹酚酸B能上调高脂饮食小鼠牙槽骨SOD的表达,下调Nox4、NF-κB-p65和Cathepsin K的表达。结论丹酚酸B可能通过调节Nox4/SOD/NF-κB/Cathepsin K通路改善高脂饮食引起的氧化应激,从而抑制高脂饮食诱发的小鼠牙槽骨骨量丢失。本研究将为丹参及其有效成分治疗骨质疏松提供科学的实验依据。
Objective To explore the effect and mechanism of salvianolic acid B on alveolar bone loss induced by high-fat diet in mice. Methods Thirty male C57BL / 6J mice were divided into three groups: normal group, high fat diet group and salvianolic acid B group, with 10 mice in each group. In addition to the normal group, the other two groups were given a high-fat diet, and the salvianolic acid B group was treated with salvianolic acid B [125 mg / (kg · d)] for 12 weeks. Bone mineral density (BMD) was measured by dual-energy X-ray and bone microarchitecture was observed by HE staining. The expressions of NF-κB-p65 and Cathepsin K in alveolar bone were detected by immunohistochemical staining. , Superoxide dismutase (SOD) and NADPH oxidase 4 (Nox4) were detected. Results Salvianolic acid B could significantly decrease the alveolar bone density induced by high-fat diet and the destruction of the bone micro-structure. Meanwhile, salvianolic acid B up-regulated the expression of SOD in alveolar bone and down-regulated the expression of Nox4, NF-κB-p65 and Cathepsin K in high fat diet mice. Conclusion Salvianolic acid B may improve the oxidative stress induced by high-fat diet by regulating Nox4 / SOD / NF-κB / Cathepsin K pathway, and thus inhibit the bone loss of alveolar bone induced by high-fat diet in mice. This study will provide a scientific experimental basis for the treatment of osteoporosis of Salvia miltiorrhiza and its active ingredients.