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目的探索利用菲立磁和转染试剂体外磁性标记食蟹猴骨髓基质细胞(BMSCs)方法的可行性。方法无菌条件下取食蟹猴骨髓,梯度密度离心法分离获取BMSCs,使用菲立磁-多聚赖氨酸复合物(FE-PLL)标记BMSCs,普鲁士蓝染色、电镜和台盼蓝排除实验等方法鉴定FE-PLL标记食蟹猴BMSCs的效率和细胞的活力,倒置相差显微镜和免疫细胞化学方法检测BMSCs的增殖和分化能力。结果菲立磁可以高效率地标记BMSCs,标记效率在99%左右。光镜和电镜下BMSCs胞质内分别可见细小的蓝色铁颗粒和许多包裹铁颗粒的囊泡。FE-PLL标记对BMSCs的活力、增殖和分化等能力没有明显的影响。结论菲立磁可以用来体外标记食蟹猴BMSCs。
Objective To explore the feasibility of in vitro magnetic labeling of cynomolgus monkey bone marrow stromal cells (BMSCs) with phenanthroline and transfection reagents. Methods BMSCs were isolated from the cynomolgus monkeys under aseptic conditions. BMSCs were isolated by gradient density centrifugation. BMSCs were labeled with FE-PLL, Prussian blue staining, electron microscopy and trypan blue exclusion And other methods to identify the efficiency and cell viability of FE-PLL labeled cynomolgus BMSCs. The proliferation and differentiation of BMSCs were detected by inverted phase contrast microscopy and immunocytochemistry. The results of Philippine magnetic labeling of BMSCs can be efficient, labeling efficiency of about 99%. Light microscopy and electron microscopy of BMSCs cytoplasm were visible in the small blue iron particles and many iron particles encapsulated vesicles. FE-PLL labeling had no significant effect on the ability of BMSCs to proliferate and differentiate. Conclusions Feridex can be used to label cynomolgus monkey BMSCs in vitro.