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目的:研究CCL25是否影响鼻咽癌CNE-2细胞的迁移。方法:采用Transwell实验,上室中加入CNE-2细胞,下室中加入不同浓度的CCL25,观察其对CNE-2向下室迁移的影响。另外同时在下室中加入anti-CCR9,再观察CNE-2向下室的迁移情况。结果:CCL25具有趋化CNE-2细胞的作用,同空白对照组相比,50ng/ml的CCL25能明显增强CNE-2细胞的迁移率(P<0.01)。而1μg/ml的anti-CCR9能逆转CCL25对CNE-2细胞的促迁移作用(P<0.05)。结论:CCL25具有趋化CNE-2细胞,促进CNE-2细胞迁移的作用。Anti-CCR9能阻断CCL25对CNE-2细胞的促迁移作用。
Objective: To investigate whether CCL25 affects the migration of CNE-2 cells. METHODS: Transwell experiments were performed in which CNE-2 cells were added into the upper chamber. Different concentrations of CCL25 were added into the lower chamber to observe the effect of CNE-2 on the migration of CNE-2 to the lower chamber. In addition, anti-CCR9 was added to the lower chamber at the same time, and then the migration of CNE-2 to the lower chamber was observed. Results: CCL25 had the chemotactic effect on CNE-2 cells. Compared with the blank control group, CCL25 at 50ng / ml significantly enhanced the CNE-2 cell migration (P <0.01). However, anti-CCR9 (1μg / ml) reversed the effect of CCL25 on CNE-2 cell migration (P <0.05). Conclusion: CCL25 has the function of chemotaxis CNE-2 cells and promote the migration of CNE-2 cells. Anti-CCR9 blocked CCL25-induced migration of CNE-2 cells.