论文部分内容阅读
目的观察天麻钩藤饮对视神经夹伤模型大鼠视网膜神经上皮层厚度和视网膜神经节细胞(RGC)凋亡的影响。设计实验研究。研究对象 SPF级雄性Wistar大鼠48只。方法将大鼠随机分6组,每组8只,分别为正常对照组,阴性对照组,天麻钩藤饮低剂量组(0.6 g/ml)、中剂量组(1.2 g/ml)、高剂量组(2.4 g/ml)和银杏叶片阳性对照组(1.2 mg/ml),除正常对照组大鼠不做处理,其他组大鼠右眼均建立视神经夹伤模型,正常对照组和阴性对照组给予纯净水灌胃。于给药30天处死动物取眼球,做石蜡切片HE染色,观察各组视网膜神经上皮层厚度,TUNEL法检测RGC的凋亡程度。主要指标 HE染色视网膜神经上皮厚度及RGC凋亡数量。结果阴性对照组(171.04±13.86μm)比正常组(208.98±8.46μm)视网膜神经上皮厚度显著减少(P=0.000)。而天麻钩藤饮中、高剂量组大鼠视网膜厚度(分别为187.68±11.16μm和189.22±9.54μm)比阴性对照组显著增加(P=0.043,0.001),且中、高剂量组与阳性对照组(191.35±9.03μm)之间无显著差异(P=0.052,0.670);TUNEL凋亡检测发现,阴性对照组凋亡细胞数(9.09±2.24个/高倍视野)比正常组(0.59±0.61个/高倍视野)明显增加(P=0.000),中剂量组、高剂量组和阳性对照组RGC的凋亡(分别为7.00±1.88,5.22±2.05,5.03±2.03个/高倍视野)均比阴性对照组显著减少(P=0.024,0.000,0.000)。结论天麻钩藤饮对大鼠视神经夹伤模型具有一定抗RGC凋亡的作用,随药物浓度的升高,抗凋亡作用更显著。
Objective To observe the effect of Tianma Gouteng Decoction on the thickness of retinal nerve epithelium and the apoptosis of retinal ganglion cells (RGCs) in optic nerve crush injury model rats. Design experiment research. Forty-eight SPF male Wistar rats were included in this study. Methods The rats were randomly divided into 6 groups with 8 rats in each group, which were normal control group, negative control group, Tianma Gouteng Yin low dose group (0.6 g / ml), middle dose group (1.2 g / (2.4 g / ml) and Ginkgo biloba positive control group (1.2 mg / ml), except for the normal control group rats were not treated, the other groups of rats were established right optic nerve injury models of optic nerve, normal control group and negative control group Give pure water gavage. The animals were sacrificed 30 days after surgery to take the eyeball, paraffin sections HE staining, the retinal nerve epithelial thickness was observed in each group, TUNEL assay RGC apoptosis. The main indicators HE staining retinal nerve epithelial thickness and the number of RGC apoptosis. Results The negative control group (171.04 ± 13.86μm) showed a significant decrease in the thickness of retinal epithelium compared with the normal group (208.98 ± 8.46μm) (P = 0.000). However, the retinal thickness (187.68 ± 11.16μm and 189.22 ± 9.54μm, respectively) of Tianma Gouteng Decoction medium and high dose groups were significantly higher than those of negative control group (P = 0.043,0.001) There was no significant difference between the two groups (191.35 ± 9.03μm) (P = 0.052,0.670). The apoptotic cells in the negative control group (9.09 ± 2.24 / high power field) / High power field) (P = 0.000). The apoptosis of RGCs in middle dose group, high dose group and positive control group were 7.00 ± 1.88, 5.22 ± 2.05 and 5.03 ± 2.03 / Group was significantly reduced (P = 0.024, 0.000, 0.000). Conclusion Tianma Gouteng Decoction has a certain anti-RGC apoptosis effect on the model of optic nerve crush in rats. With the increase of drug concentration, anti-apoptotic effect is more significant.