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切碎猪卵巢,用胶原酶酶解分离,经尼龙筛过筛后可得到大量带透明带的卵子。每个卵巢可取得200多个卵子,没有颗粒细胞和碎片粘附于透明带。将猪透明带移入磷酸缓冲液中,在76°—78°C加热30分钟制成抗原。此抗原免疫了九只雌免,都产生专一性抗血清。用间接萤光法和免疫扩散法检测抗血清。间接萤光法测定抗血清滴度范围在1:2000～4000。对兔、羊、牛、猕猴的透明带也有强烈作用。这种抗血清在琼脂免疫扩散板上,与猪和兔的各种组织盐浸液之间均不呈现沉淀线。透明带免疫后的九只雌兔,在动情期多次交配,均未受孕。
Chop porcine ovary, with collagenase enzymatic separation, sieved through a nylon mesh can be obtained with a large number of eggs with zona pellucida. Each ovary can be obtained more than 200 eggs, no particles and debris attached to the zona pellucida. The zona pellucida was transferred to phosphate buffer and heated at 76 ° -78 ° C for 30 minutes to make antigen. This antigen immunized nine female immunization, have produced specific antiserum. Antisera were detected by indirect fluorescence and immunodiffusion assays. Indirect fluorescence assay antiserum titers in the range of 1: 2000 ~ 4000. It also has a strong effect on the zona pellucida of rabbits, sheep, cattle and macaques. This antiserum showed no precipitation line between agar immunol diffusion plates and various tissue salt infusions from pigs and rabbits. Nine female rabbits after immunization with zona pellucida were repeatedly mated during estrus, and were not pregnant.