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应用胎肌浸液(HM—CM)和植物血疑素白细胞条件培养液(PHA—LCM),对35例慢性粒细胞白血病患者的骨髓细胞进行体外培养,观察其集落增殖状况和细胞演变过程.结果显示:慢性期HM—CM体系集落数异常增高,高于PHA—LCM体系和正常对照组,且HM—CM集落数多少与慢性期的长短呈负相关,集落内均为中晚幼粒细胞.加速期的特点为PHA—LCM体系的集落数逐渐升高,而HM—CM体系的集落数下降,集落内原始细胞为14.1％±3.2％和8.0％±1.8％.急变期与急粒组相似,PHA—LCM体系集落旺盛,集落内细胞均由原始细胞组成,HM—CM体系则很少生长集落.体外实验诊断慢粒急变要比临床提前2～9个月出现,因此用该法预测慢粒急变有一定的价值。
The bone marrow cells of 35 patients with chronic myelocytic leukemia were cultured in vitro using fetal muscle infusion (HM-CM) and phytopathogenic white blood cell conditioned medium (PHA-LCM) to observe the colony proliferation and cell evolution. The results showed that the number of colonies in the chronic HM-CM system increased abnormally and was higher than that in the PHA-LCM system and the normal control group. The number of HM-CM colonies was negatively correlated with the length of the chronic phase, and all of the colonies were medium and late myelocytes. The accelerating phase is characterized by a gradual increase in the number of colonies in the PHA-LCM system and a decrease in the number of colonies in the HM-CM system, with 14.1% ± 3.2% and 8.0% ± 1.8% of the original cells in the colonies. Similarly, the PHA-LCM system colonies are prosperous, the colony cells are composed of primitive cells, and the HM-CM system rarely grows colonies. The in vitro experimental diagnosis of chronic myeloid emerges 2 to 9 months earlier than the clinical one, so this method is used to predict Slow grain change has a certain value.