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目的:观察山莨菪碱对离体正常(Wistar)大鼠及自发性高血压大鼠(spontaneously hypertensive rats,SHR)腹主动脉舒张作用的影响,并探讨其可能的作用机制。方法:采用大鼠离体腹主动脉环(长度为4~5mm的血管)灌流技术,观察山莨菪碱对正常大鼠及SHR血管舒张功能的影响,并观察苯肾上腺素(phenylephrine,PE)、左旋硝基精氨酸甲酯(L-NAME)预处理对山莨菪碱作用的影响。结果:山莨菪碱对PE预收缩的正常大鼠内皮完整和去内皮血管的舒张作用差异显著(P<0.05);对PE预收缩的SHR内皮完整及去内皮血管环,山莨菪碱均具有显著舒张作用,呈剂量-效应关系,最大舒张率分别为(78.6±6.9)%和(65.76±11.39)%,无统计学差异。用NOS抑制剂L-NAME预处理正常大鼠内皮完整的血管环,可显著地抑制山莨菪碱诱导的舒张作用(P<0.05)。山莨菪碱对SHR血管的舒张作用能够被L-NAME抑制,抑制前后最大舒张率分别为61%和11.9%(P<0.01)。结论:山莨菪碱可能通过内皮和平滑肌2条途径发挥舒张大鼠离体腹主动脉的作用。
Objective: To observe the effect of anisodamine on the diastolic function of abdominal aorta in isolated Wistar rats and spontaneously hypertensive rats (SHR), and to explore its possible mechanism. Methods: The effects of anisodamine on the vasodilation function of normal rats and SHR were observed by perfusion of isolated abdominal aortic rings (4 ~ 5mm in length) in rats. Phenylephrine (PE) Effect of L-NAME pretreatment on anisodamine. RESULTS: Anisodamine showed significant difference in the integrity of endothelium and endothelium of normal rats pre-contracted by PE (P <0.05), and there was significant difference between intact endothelium of PE with pre-contracted PE and endothelium-dented ring and anisodamine There was no significant difference between the two groups in the dose-effect relationship and the maximum diastolic rate (78.6 ± 6.9)% and (65.76 ± 11.39)%, respectively. NOS inhibitor L-NAME pretreatment of normal rat endothelial integrity of the vascular ring, can significantly inhibit anisodamine-induced diastolic (P <0.05). Anisodamine attenuated SH-induced vasorelaxation by L-NAME, with maximal relaxation rates of 61% and 11.9%, respectively (P <0.01). Conclusion: Anisodamine may play a role in relaxing isolated abdominal aorta in rats by two pathways of endothelium and smooth muscle.