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目的检测血管内皮生长因子(vascular endothelial growth factor,VEGF)及其受体(VEGF receptor,VEGFR)在稽留流产(missed abortion,MA)患者及正常孕妇绒毛组织中的表达,探讨其在稽留流产的发病机制及临床的意义。方法选择深圳市龙岗中心医院2015年2月—9月MA患者96例,试验组1(原因明确)51例、试验组2(原因不明)45例。取同期孕妇57例作为对照组。采用免疫组化方法测定绒毛组织中VEGF、VEGFR-1、VEGFR-2的表达,用平均灰度值(OD值)表示。结果试验组1、试验组2患者绒毛组织中VEGF、VEGFR-1和VEGFR-2的OD值分别为33.08±3.21、35.13±4.05、34.60±4.14和37.69±3.78、36.21±4.13、36.94±4.09,均低于对照组,试验组1 VEGF的OD值低于试验组2,差异有统计学意义(P<0.05)。试验组1、试验组2患者绒毛组织中VEGF、VEGFR-1和VEGFR-2的阳性率分别为29.41%、33.33%、31.37%和37.77%、31.11%、33.33%,均低于对照组,且试验组1 VEGF阳性率低于试验组2,差异有统计学意义(P<0.05)。试验组1 VEGF/VEGFR-1为0.88,低于试验组2,差异有统计学意义(P<0.05)。结论 MA患者绒毛组织中VEGF及其受体VEGFR-1、VEGFR-2的表达呈现低表达,原因明确患者的VEGF表达低于不明确患者,且VEGF/VEGFR-1降低,导致MA发生的机制可能与胚胎血管生成不足有关。
Objective To detect the expression of vascular endothelial growth factor (VEGF) and its receptor (VEGFR) in villi of patients with missed abortion (MA) and normal pregnant women, and to explore its role in the development of missed abortion Mechanism and clinical significance. Methods 96 cases of MA patients in Shenzhen Longgang Central Hospital from February to September in 2015, 51 cases in experimental group 1 (definite cause) and 45 cases in experimental group 2 (unexplained). Take the same period 57 pregnant women as a control group. Immunohistochemistry was used to determine the expression of VEGF, VEGFR-1 and VEGFR-2 in chorionic villi and expressed as mean gray value (OD value). Results The OD values of VEGF, VEGFR-1 and VEGFR-2 in villus of experimental group 1 and experimental group 2 were 33.08 ± 3.21, 35.13 ± 4.05, 34.60 ± 4.14 and 37.69 ± 3.78, 36.21 ± 4.13 and 36.94 ± 4.09 respectively, The OD value of VEGF in experimental group 1 was lower than that in experimental group 2, the difference was statistically significant (P <0.05). The positive rates of VEGF, VEGFR-1 and VEGFR-2 in villi of experimental group 1 and experimental group 2 were 29.41%, 33.33%, 31.37% and 37.77%, 31.11% and 33.33% respectively, which were lower than that of control group The positive rate of VEGF in experimental group 1 was lower than that in experimental group 2, the difference was statistically significant (P <0.05). The experimental group 1 VEGF / VEGFR-1 was 0.88, lower than the experimental group 2, the difference was statistically significant (P <0.05). Conclusions The expression of VEGF and VEGFR-1 and VEGFR-2 in villi of MA patients is low. The reason for this is clear that the expression of VEGF in patients with lower MA is lower than that in patients with unknown, and the VEGF / VEGFR-1 is decreased, which may result in the possible mechanism of MA Related to lack of embryo angiogenesis.