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目的:研究天麻素对模拟高原缺氧大鼠心脏的保护作用。方法:2月龄雄性Wistar大鼠60只,按随机数字表法随机分为常氧模型组、缺氧模型组、红景天(阳性对照)组、天麻素(35、75、105mg/kg)组,每组10只。每日定时灌胃,连续7天。采用低压氧舱模拟海拔8000 m高原环境,将除常氧模型组外的5组大鼠置入实验舱,缺氧12h后观察大鼠心组织病理显微结构的改变,并测定心组织相关生化指标水平。结果:与常氧模型组相比,缺氧12h后,缺氧模型组大鼠心组织出现病理损伤,心组织中乳酸(LD)含量、乳酸脱氢酶(LDH)活性、丙二醛(MDA)含量、过氧化氢(H2O2)含量显著上升,谷胱甘肽过氧化物酶(GSH-Px)活力显著下降。与缺氧模型组相比,天麻素(35、75、105mg/kg)组心组织病理损伤较小;天麻素(35、105mg/kg)组LD含量显著下降且与常氧模型组无差异,天麻素75mg/kg组LD含量显著下降但与常氧模型组有差异;天麻素(35、75mg/kg)组LDH活性有下降趋势但无统计学意义仍高于正常,天麻素105mg/kg组LDH活性有显著下降与常氧模型组无差异;天麻素(35、75、105mg/kg)组MDA含量、H2O2含量均显著下降,GSH-Px活性显著上升,且均与常氧模型无差异。结论 :研究表明天麻素对模拟高原缺氧大鼠心脏具有保护作用。
Objective: To study the protective effect of gastrodin on the heart of hypoxia rats. Methods: Sixty male Wistar rats aged 2 months were randomly divided into normoxia model group, hypoxia model group, Rhodiola (positive control group), gastrodin (35, 75, 105 mg / kg) Group, 10 in each group. Daily regular gavage for 7 days. Using hypobaric oxygen chamber to simulate the altitude of 8000 m plateau, five rats except the normoxia model group were placed in the experimental cabin, and the changes of the pathological microstructure of the rat heart were observed after hypoxia for 12 hours. Indicator level. RESULTS: Compared with normoxia model group, the pathological changes of heart tissue were observed in hypoxia model rats after 12 hours of hypoxia. The levels of lactate (LD), lactate dehydrogenase (LDH), malondialdehyde (MDA) ), The content of hydrogen peroxide (H2O2) increased significantly and the activity of glutathione peroxidase (GSH-Px) decreased significantly. Compared with the hypoxia model group, gastrodin (35, 75, 105 mg / kg) had less pathological damage in the heart tissue; LD decreased significantly in gastrodin (35, 105 mg / kg) group and no difference with the normoxia model group Gastrodin 75mg / kg group LD decreased significantly but with the normoxic model group differences; Gastrodin (35,75mg / kg) LDH activity decreased but no statistically significant higher than normal gastrodin 105mg / kg group LDH activity was significantly decreased compared with normoxia model group; gastrodin (35,75,105mg / kg) group MDA content, H2O2 content were significantly decreased, GSH-Px activity increased significantly, and no difference with the normoxia model. Conclusion: The study shows that gastrodin has a protective effect on the hypoxic rat heart.