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目的:诱导叶下珠得到愈伤组织并对其培养条件进行优化。方法:以茎、叶、种子为外植体,以MS为基本培养基,考察不同外植体在不同外源激素及不同配比下愈伤组织的诱导率;以诱导条件下优化得到的激素配比为基础,通过改变激素NAA、2,4-D和6-BA的相对比例,考察叶下珠愈伤组织继代生长的最佳条件。结果:茎为最佳外植体;最佳诱导培养基为MS+0.1 mg/L NAA+0.1 mg/L 2,4-D+2 mg/L 6-BA,此条件下诱导率可达90%以上;最佳继代培养基为MS+0.5 mg/L NAA+0.5 mg/L 2,4-D+2 mg/L 6-BA;外源激素NAA、2,4-D和6-BA不同比例组合后对叶下珠愈伤组织诱导及继代培养影响较大。结论:首次成功建立叶下珠愈伤组织快速培养体系,为利用现代生物技术方法保护和利用叶下珠物种资源提供了一种新途径。
OBJECTIVE: To induce callus from Phyllanthus urinaria and optimize its culture conditions. Methods: The stems, leaves and seeds were used as explants and MS as the basic medium. The induction rate of different explants under different exogenous hormones and different proportions was investigated. The optimal hormone Based on the ratio, the optimum conditions of subcultures of Phyllanthus urinaria were investigated by changing the relative proportions of hormones NAA, 2,4-D and 6-BA. Results: The stems were the best explants. The optimal induction medium was MS + 0.1 mg / L NAA + 0.1 mg / L 2,4-D + 2 mg / L 6-BA. %. The best subculture medium was MS + 0.5 mg / L NAA + 0.5 mg / L 2,4-D + 2 mg / L 6-BA; exogenous hormones NAA, 2,4-D and 6-BA Different proportion combination of Phyllanthus callus callus induction and subculture greater impact. Conclusion: The rapid culture system of Phyllanthus euphratica callus was successfully established for the first time, which provided a new way to protect and utilize Phyllanthus species with modern biotechnology methods.